Fig. 8: CPI-613 reduces tumor burden and invasion in zebrafish xenografts of DLST-dependent but not independent TNBC cells.

a The representative overlay images of brightfield and RFP channel of zebrafish embryos transplanted with RFP+ BT-549 cells at 2 days of post-treatment. b, c Quantitative data indicate the changes of tumor invasion (b) and burden (c) after the treatment. Tumor cell invasion was determined for the percentage of zebrafish xenografts that have tumor cells migrated away from the original injection site in each experimental repeat (a, b): n = 3–6. Tumor burden was quantified based on the fluorescence intensity using ImageJ 1.52a (a, c). Fish number for BT-549: n = 27 for vehicle and 20 for CPI-613, respectively; and Hs578T: n = 24 for vehicle and 22 for CPI-613, respectively. Scale bar = 0.5 mm. Statistical difference was calculated using an unpaired two-tailed t-test. *P ≤ 0.05, ***P ≤ 0.001.