Fig. 1: Fasting-induced Torpor Shows a Reversible Transcriptome Signature.

a Protocol for sampling muscles from Pre, Mid, and Post torpor animals to test the reversibility of the transcriptional profile of muscles during torpor. b Boxplots for the VO₂ of animals at sampling in the reversibility experiment #1. Each dot represents one sample from one animal. During torpor (Mid group), the median VO₂ was lower than during Pre or Post torpor. The band inside the box, the bottom of the box, and the top of the box represent the median, the first quartile (Q₁), and the third quartile (Q₃), respectively. The interquartile range (IQR) is defined as the difference between Q₁ and Q₃. The end of the lower whisker is the lowest value still within 1.5 IQR of Q₁, and the end of the upper whisker is the highest value still within 1.5 IQR of Q₃. Every other boxplot in this manuscript follows the same annotation rules. The numbers in the Mid torpor group are identification numbers of the animals. c MDS plot of the TSS-based distance in reversibility experiment #1. Each dot represents one sample from one animal. The Mid group clustered differently from the Pre and Post groups in the 1st dimension. The two internal groups seen in the Mid group in d were not evident in this plot, indicating the transient metabolic change during torpor was not correlated with transcription. d Hierarchical clustering heatmap based on the TPM of TSS detected in the reversibility experiment #1. The group colors are denoted in (c). e Distribution of CAGE clusters according to the fold-change (FC) in the TPM of Pre to Mid and Mid to Post torpor. The top five up- and down-regulated reversible promoters that had annotated downstream genes are shown. f Top ten enriched GO terms in the reversible promoters. g Top ten enriched KEGG pathways in the reversible promoters. h, i Top five up- and down-regulated reversible promoters ordered according to the magnitude of the TPM change. Promoters that had annotated downstream genes are shown.