Fig. 2: Characterization of telomere lengths, telomere ssDNA, and telomerase activity in matched ADRN and MES cell lines. | Communications Biology

Fig. 2: Characterization of telomere lengths, telomere ssDNA, and telomerase activity in matched ADRN and MES cell lines.

From: Reciprocal impacts of telomerase activity and ADRN/MES differentiation state in neuroblastoma tumor biology

Fig. 2

a The telomeres of the indicated cell lines were subjected to TRF Southern analysis. b The telomeres of the indicated cell lines were subjected to STELA analysis. Extra-short telomeres (<1 Kb) are demarcated by horizontal red lines across the panels. c The STELA fragments from each cell line in b (n = 6 PCR reactions) were analyzed using TeSLA software and the pooled results presented as violin plots with medians and quartiles. d The amount of ssDNA in the telomere G- and C-strand in the indicated cell lines were analyzed by native in-gel hybridization. e Telomerase activities in the indicated cell lines were analyzed by TRAP and plotted (means, n = 2 independent extracts).

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