Fig. 4: Profiling of BE(2)N cells during Dn-hTERT-induced switch from ADRN to MES cell types.

a TRAP analysis of telomerase activity in BE(2)N harboring either hTERT or Dn-hTERT at different time points following retrovirus infection. The assays were performed using serial dilutions of the extracts as indicated. The relative activity of each extract was quantified using ImageQuant and plotted at the bottom (mean ± S.D., n = 2 or 3 experimental replicates). Pair-wise P-values were determined using student’s t-tests (two-tailed). b Analysis of telomere length distributions in BE(2)N harboring either hTERT or Dn-hTERT at different time points following retrovirus infection. c Western analysis of telomere and lineage-related proteins in BE(2)N during passage of cells infected with viruses that harbor either hTERT or Dn-hTERT. d The number of upregulated and downregulated genes in Dn-hTERT-treated BE(2)N at the indicated time points (by >2-fold in comparison to the DMSO-treated control cells) were determined from RNA-seq analysis and plotted. e The expression patterns of the ~1000 genes that show the greatest changes in mRNA levels (>2 fold) in BrdU-treated BE(2)N were displayed using Heatmap. f Pair-wise comparison of the degrees of overlaps between genes upregulated by Dn–hTERT treatment at different time points. g Pair–wise comparison of the degrees of overlaps between genes downregulated by Dn-hTERT treatment at different time points.