Fig. 1: Purification of AcrB in different stabilising systems. | Communications Biology

Fig. 1: Purification of AcrB in different stabilising systems.

From: Cycloalkane-modified amphiphilic polymers provide direct extraction of membrane proteins for CryoEM analysis

Fig. 1

SDS-PAGE of purification of AcrB in a DDM, b SMA, c A8-35, d C6-C2-50, and e C8-C0-50. Purification is shown with flow-through (FT) from affinity beads, and wash (W) steps, in addition to soluble (S) and insoluble (I) samples for the amphipol purifications (for experimental details, see the M&M section). All polymers showed the ability to directly extract and purify AcrB with different efficiencies and resulting in different purities. The CyclAPols C6-C2-50 and C8-C0-50 showed a reduced efficiency to DDM and SMA (mainly because their concentration used at the solubilisation step is ten times lower) but with much-improved efficiency than the A8-35 polymer.

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