Fig. 2: Negative stain electron microscopy of AcrB extracted and purified in SMA, A8-35 and CyclAPols. | Communications Biology

Fig. 2: Negative stain electron microscopy of AcrB extracted and purified in SMA, A8-35 and CyclAPols.

From: Cycloalkane-modified amphiphilic polymers provide direct extraction of membrane proteins for CryoEM analysis

Fig. 2

Representative micrographs were collected at 49k magnification of AcrB following purification in (a) SMA, (b) A8-35, (c) C6-C2-50, and (d) C8-C0-50. Scale bar is 100 nm in all images. Negative stain 2D classes of AcrB in (e) C6-C2-50 and (f) C8-C0-50 are also shown with some representative high-angle classes highlighted in green. Images (a) and (b-d) were taken using Tecnai F20 and G2-spirit T12 transmission electron microscopes, respectively.

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