Fig. 3: CryoEM of AcrB extracted and purified in CyclAPol C₈-C₀-50.

a Classes of AcrB in C₈-C₀-50 following one round of 2D classification. The classes demonstrate a range of views such as a high angle view showing putative threefold symmetry (highlighted in green) with classes showing clear doublets highlighted in red. b Side view of the AcrB cryoEM map at 3.2 Å final resolution. c Representative map density of AcrB in C₈-C₀-50 around the top of the vestibule region. d Overlay of the cryoEM structure of AcrB in SMA⁴⁰ (PDB accession code: 6baj; pink) with the AcrB in C₈-C₀-50 structure (PDB accession code 7B5P; grey) demonstrating the close similarity of structures.