Fig. 5: Cross-reactivity analysis across coronaviruses reveals shared epitopes and epitopes specific to SARS-CoV-2. | Communications Biology

Fig. 5: Cross-reactivity analysis across coronaviruses reveals shared epitopes and epitopes specific to SARS-CoV-2.

From: High-resolution epitope mapping and characterization of SARS-CoV-2 antibodies in large cohorts of subjects with COVID-19

Fig. 5

PIWAS was performed using COVID-19 samples against various coronavirus proteomes including SARS-CoV-2, SARS, MERS, and the common hCoVs HKU1, OC43, 229E, and NL63. PIWAS tilings for a spike and d nucleoprotein revealed regions of cross-reactivity as well as epitopes only observed against SARS-CoV-2. Clustal multiple sequence alignments were performed and visualized below to depict sequence similarity and divergence. Distinct epitopes from b spike and e nucleoprotein showcase PIWAS values across the coronaviruses with corresponding clustal alignment sequences below. Epitope locations are denoted with asterisks in a and d. Distribution of PIWAS values at epitopes from spike c and nucleoprotein f for severe (n = 447), moderate (n = 134), and mild (n = 170) cases, with p-values from Wilcoxon-rank sum test.

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