Fig. 5: Increased cytokines and chemokines in APPswe+ OB-progenitor cells. | Communications Biology

Fig. 5: Increased cytokines and chemokines in APPswe+ OB-progenitor cells.

From: Osteoblastic Swedish mutant APP expedites brain deficits by inducing endoplasmic reticulum stress-driven senescence

Fig. 5

a Schematic of purification and RNA-seq of Tdtomato+ (Td+) OB progenitors from control (OCN-Cre; Ai9) and TgAPPsweOCN; Ai9 mice. b–d Volcano plots (b), GO analysis of up/down-regulated genes (c), and heat map (d) of differentially expressed genes identified by RNA-seq. e RT-PCR analysis of AD risk gene App, Vps35, Trem2, Apoe, Ptk2b, and Sorl1; bone-mass regulator Sp7, Nfatc1, Col1a1, Spp1, Sparc, Bmp2, Lrp4, and Ctnnb1; cytokine Il1b, Il6, and Il10; chemokine Ccl5 and Cxcl1, growth factor Tgfb1 gene expression in purified Td+ OB progenitors from 6-MO control (OCN-Cre; Ai9) and TgAPPsweOCN; Ai9 mice. All values were presented as mean ± SD (n = 3 mice). *p < 0.05, **p < 0.01, and ***p < 0.001, by Mann–Whitney U test.

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