Fig. 9: APP_swe induction of OB-senescence via ER stress.

a Heat map of differentially expressed ER stress or anti-stress related genes identified by RNA-seq in control (OCN-Cre; Ai9) and TgAPP_swe^OCN; Ai9 Td⁺ OB-progenitors (detail analysis was described in Methods). b RT-PCR analysis of ER stress-related genes Grp78, Atf6, Hsp90b1, Eif2ak3, Ern1, Hsp90aa1, and Hspa2 and anti-stress related gene Sirt3 gene expression in purified Td⁺ BMSCs from 6-MO control (OCN-Cre; Ai9) and TgAPP_swe^OCN; Ai9 mice, *p < 0.05, **p < 0.01, ***p < 0.001, mean ± SD, n = 3, Mann–Whitney U test. c Western blot analysis of indicated protein expression in BMSCs from mice with indicated genotypes (at 6-MO). GAPDH was used as a loading control. d Quantification of data in c, *p < 0.05, **p < 0.01. mean ± SD, n = 4, Student’s t test. e Western blot analysis of indicated protein expression in BMSCs from 6-MO control and TgAPP_swe^OCN with or without 0.25 mM 4-PBA (4-Phenylbutyric acid) treatment. f Quantification analyses of the data in e, *p < 0.05, n = 3. g SA-β-gal staining of 6-MO control and TgAPP_swe^OCN BMSCs with vehicle (Veh)(PBS) and 4-PBA treatment, respectively, scale bar, 20 µm. h Quantification of SA-β-gal⁺ cell densities in g (mean ± SD; n = 5, **p < 0.01, ***p < 0.001). Two-way analysis of variance test was used in f and h.