Table 2 Genes responsible for the synthesis of DHA found in the draft genomes of A. limacinum, T. aureum, and Parietichytrium sp.

From: PUFA synthase-independent DHA synthesis pathway in Parietichytrium sp. and its modification to produce EPA and n-3DPA

  

A. limacinum Type I

T. aureum Type II

Parietichytrium sp. Type III

FAS

Query seq.

E-value

Schizochytrium type I FAS

0a

Schizochytrium type I FAS

2e−178a

Schizochytrium type I FAS

0a

PUFA-S

Query seq.

E-value

Schizochytrium PUFA-S A/B/C

0a/0a/0a

Schizochytrium PUFA-S A/B/C

0a/0a/0a

Schizochytrium PUFA-S A/B/C

5e−43b/2e−25c/not foundd

Δ4DES

Query seq.

E-value

T. aureum Δ4DES

not foundd

T. aureum Δ4DES

0a

T. aureum Δ4DES

0a

  1. Draft genome databases of A. limacinum ATCC MYA-1381 (provided by the U.S. Department of Energy, Joint Genome Institute, USA), T. aureum ATCC34304 (this study), and Parietichytrium sp. I65-24A (this study) were searched using amino acid sequences of type I FAS (EF015632) from Schizochytrium sp. ATCC 20888, PUFA-S subunit A (AF378327), subunit B (AF378328), subunit C (AF378329) from Schizochytrium sp. ATCC 20888, and Δ4DES from T. aureum ATCC 34304 (AF391543). The E-value of each gene is described in the table.
  2. aA corresponding sequence was found in each database.
  3. b,cA homologous sequence was found in the Parietichytrium database, but the highest homologous sequence was found in bMarinagarivorans algicola as “hybrid nonribosomal peptide synthetase/type I polyketide synthase” (E-value = 0.0) or cArabidopsis thaliana as “3-oxoacyl carrier protein synthase” (E-value = 1e−116).
  4. dHomologous sequences (E-value < 10) were not found in each database.