Fig. 1: PEPD binds directly to p53 mutants.
From: Loss of peptidase D binding restores the tumor suppressor functions of oncogenic p53 mutants
a Recombinant human PEPD (10–2560 nM) was incubated with recombinant human p53 or p53 mutants (250 nM) overnight at 4 °C. Their binding was measured by ELISA. Each value is mean ± SD (n = 3). b Recombinant human PEPD (801 nM) and p53 (114 nM) or p53R175H (114 nM) were incubated alone or with each other for 15 min at room temperature, then incubated with BS3 (a cross-linker) for 30 min at room temperature, and analyzed by WB for PEPD, p53 and p53R175H. p53 was included in each experiment for comparison.
