Fig. 5: PEPD KD reactivates the transcription-independent tumor suppressor activities of p53 mutants.
From: Loss of peptidase D binding restores the tumor suppressor functions of oncogenic p53 mutants
a Cells were treated by siRNA (10 nM) for 48 h. p53, p53 mutants and other proteins in subcellular fractions were analyzed by WB, using voltage-dependent anion channel (VDAC), GAPDH and lamin B as loading controls. b Cells were treated by siRNA (10 nM) for 48 h. MMP was measured by JC-1 fluorescence. c Cells were treated by siRNA (10 nM) for 48 h, from which mitochondria were isolated and analyzed by IP-WB for CYPD binding to p53 mutants. d Cells were treated by siRNA (10 nM) for 72 h. Apoptosis was measured by TUNEL assay, counting 500 cells per sample (see also Supplementary Fig. 5). The bar-dot plots in b and d show individual values and mean ± SD (n = 3). ****P < 0.0001, by paired two-tailed t-test in b. Cells carrying p53 (MCF-7 cells and CAL-51 cells) or MDA-MB-231 (p53KO) cells were included in a, b, and d for comparison.
