Fig. 6: PEPD KD reactivates the transcription-dependent tumor suppressor activities of p53 mutants.
From: Loss of peptidase D binding restores the tumor suppressor functions of oncogenic p53 mutants
a Cells were treated by siRNA (10 nM) for 24 h. Phospho-p53 and phospho-p53 mutants in WCL were analyzed by WB. GAPDH is a loading control. b Cells were treated by siRNA (10 nM) for 48 h. Phospho-p53 and phospho-p53 mutants in subcellular fractions were analyzed by phostag WB. c Cells were transfected with an equal amount of PG13-Luc or MG15-Luc along with pRL-TK and 24 h later treated with siRNA (10 nM) for 48 h. Luciferase activity was measured in WCL. d Cells were treated by siRNA (10 nM) for 48 h. Binding of p53 mutants to the p53-binding sites in the promoters of CDKN1A and BBC3 genes were measured by ChIP-qPCR assay. The bar-dot plots in c and d show individual values and mean ± SD (n = 3). ****P < 0.0001, by paired two-tailed t-test. Cells carrying p53 (MCF-7 cells and CAL-51 cells) or MDA-MB-231 (p53KO) cells were included in a–c for comparison.
