Fig. 8: p53 mutants refold once separated from PEPD.
From: Loss of peptidase D binding restores the tumor suppressor functions of oncogenic p53 mutants
a Recombinant p53 and p53R175H were subjected to IP by PAb1620 or PAb240, followed by WB analysis of the precipitate for p53 and p53R175H. b–f WCL of untreated cells in b, c, and e or WCL of cells treated with siRNA (10 nM) for 48 h in d and f were subjected to IP by PAb1620, PAb240, or isotype-matched IgG, followed by WB analysis of the precipitate for p53 mutants and PEPD. A p53-containing sample (WCL of untreated CAL-51 cells) was used as a positive control during WB in b. The relative level of each p53 mutant in d–f was measured by ImageJ.
