Fig. 4: Potential for expanding the 16-plex reporter cell line panel.
From: A cell-based multiplex immunoassay platform using fluorescent protein-barcoded reporter cell lines
a Generation of reporter cell lines expressing a fifth FP, LSSmOrange. Reporter cell lines expressing none (FP0000) or four (FP1111) FPs in the basic panel were transduced to express LSSmOrange and monoclonal cell lines were generated. The parental cell lines FP0000 and FP1111 were designated as FP00000 and FP01111, respectively, and the derivative cell lines expressing LSSmOrange as FP10000 and FP11111, respectively. Dash lines in red indicate similar express levels of FPs between different cell lines. b The vast majority of puromycin-resistant transductants expressed surface CCR5 and the expression levels were comparable among different reporter cell lines with or without a fifth FP. The four reporter cell lines generated above were transduced to express human CCR5. After puromycin selection, the bulk transductants were detected for cell surface express of CCR5. CCR5+ populations were gated and the expression levels were compared in overlaid histogram plot on the right.
