Fig. 4: Category-specific changes in neuronal activity.
From: Long-term dynamics of aberrant neuronal activity in awake Alzheimer’s disease transgenic mice
a, b Alluvial plots depicting the fractional change between time points of highly (>4 transients/min), intermediately (0.25–4 transients/min) and rarely active (<0.25 transients/min) neurons in WT (a) and APPPS1 (b) mice over time (fraction highly active neurons: week 0–4 effect of group: F1,72 = 6.96, p = 0.012, effect of time: F2,72 = 0.15, p = 0.86, group-by-time interaction effect: F2,72 = 0.83, p = 0.44; intermediately active cells: week 0–4 effect of group: F1,72 = 5.52, p = 0.024, effect of time: F2,72 = 3.47, p = 0.036, group-by-time interaction effect: F2,72 = 0.006, p = 0.45; rarely active cells week 0–4 effect of group: F1,72 = 3.9, p = 0.056, effect of time: F2,72 = 5.24, p = 0.0075, group-by-time interaction effect: F2,72 = 0.51, p = 0.6, WT n = 9 (5 mice), APPPS1 n = 29 experiments (9 mice), two-way repeated measures ANOVA). c–e Activity-category specific changes in neuronal activity throughout the 4-week investigation period. c Highly active neurons in APPPS1 mice underwent a smaller reduction in activity than those neurons in their WT littermates (p = 0.046 for week 0–2; p = 0.012 for week 0–4, KS test, WT n = 81 neurons, APPPS1 n = 444 highly active neurons). d Intermediately active neurons increased their activity on average in APPPS1 compared to WT (p < 10−4 for week 0–2; p < 10−7 for week 0–4, KS test, WT n = 309 neurons, APPPS1 n = 951 intermediately active neurons). e Activity change of rarely active neurons did not differ between APPPS1 and WT mice (p = 0.77 week 0–2; p = 0.24 week 0–4, KS test, WT n = 94 neurons, APPPS1 n = 177 rarely active neurons). f Reoccurrence rate over four weeks of highly (p = 0.025), g intermediately and (p = 0.019) h of rarely active neurons (p = 0.1, all Mann Whitney U test, data are median ± 95% CI), *P < 0.05, **P < 0.01, ***P < 0.001.
