Fig. 6: Increased pairwise activity correlation in APPPS1 transgenic mice.
From: Long-term dynamics of aberrant neuronal activity in awake Alzheimer’s disease transgenic mice
a Representative example of a raster plot depicting binarized neuronal activity (upper panel) in both a WT and an APPPS1 mouse. Pairwise correlations (Pearson’s correlation coefficient R) were computed and sorted at the first time point and displayed as color-coded correlogram for each time point (bottom of each panel) for the WT (upper panel) and the APPPS1 (lower panel) mouse. b Average neuronal correlation in individual experiments in WT (black) and APPPS1 (red) mice for actual experimental (bright color bars) and shuffled (pale bars) data. The Pearson’s correlation coefficient (R) was significantly increased in APPPS1 mice compared to WT mice (WT vs APPPS1: effect of group: F1,72 = 6.44, p = 0.015, time: F2,72 = 0.97, p = 0.38, group-by-time interaction: F2,72 = 0.57, p = 0.57; WT vs WT shuffled, group: F1,72 = 43.66, p < 10−9, time: F2,72 = 0.18, p = 0.83, group-by-time interaction: F2,72 = 0.08, p = 0.93; APPPS1 vs APPPS1 shuffled, group: F1,72 = 167.52, p < 10−9, time: F2,72 = 1.41, p = 0.25, group-by-time interaction: F2,72 = 1.19, p = 0.31, two-way repeated measures ANOVA, WT n = 9 (5 mice), APPPS1 n = 29 experiments (9 mice)). c Average pairwise correlations during stationary (stat) and whisking (whisk) epochs in WT and APPPS1 mice (WT stationary vs WT whisking: effect of group F1,32 = 0.46, p = 0.51, WT stat n = 9 and WT whisk n = 9 experiments (5 mice); APPPS1 stationary vs APPPS1 whisking: group: F1,108 = 3.77, p = 0.057, APPPS1 stat n = 29 and APPPS1 whisk n = 29 experiments (9 mice); WT stat vs APPPS1 stat: group: F1,72 = 6.06, p = 0.019, time: F2,72 = 1.08, p = 0.34, group-by-time interaction: F2,72 = 0.42, p = 0.66; WT whisking vs APPPS1 whisking: group: F1,68 = 5.21, p = 0.029; time: F2,68 = 0.16, p = 0.86, group-by-time interaction: F2,68 = 0.49, p = 0.61, all two-way repeated measures ANOVA). d Pairwise correlations during stationary epochs between highly active (p < 10−16, WT n = 3198 neuronal pairs, APPPS1 n = 37132 neuronal pairs) and intermediately active neurons (p < 10−37, Mann–Whitney-U test, WT n = 36970 neuronal pairs, APPPS1 n = 99759 neuronal pairs). e Neuronal correlation as a function of distance between neuronal pairs in WT (black) and APPPS1 (red) mice for three imaging time points (week 0: effect of group F1,2 = 21.4, p = 10−5, distance: F2,9 = 7.42, p = 10−8, week 2: group F1,239 = 51.12, p = 10−11, distance: F9,239 = 10.17, p = 10−12, week 4: group F1,239 = 32.76, p = 10−7, distance: F9,239 = 7.41, p = 10−9, two-way ANOVA, WT n = 7 experiments, APPPS1 n = 23 experiments, only experiments with pairwise distances covering the whole range of distance bins were considered). Solid line represents an exponential fit for visual guidance. At all imaging time points we found higher pairwise correlations in APPPS1 at all neuronal distances, (data in b, c, d, e is mean ± SEM). *P < 0.05).
