Fig. 1: Comparison of HDX of His6-Hsp70 WT at pH 5.3 and 7.4. | Communications Biology

Fig. 1: Comparison of HDX of His6-Hsp70 WT at pH 5.3 and 7.4.

From: Conformational dynamics of free and membrane-bound human Hsp70 in model cytosolic and endo-lysosomal environments

Fig. 1

Difference plot illustrating the difference in HDX between pH 5.3 and 7.4 over the measured time points (cyan line: 5 s, orange line: 15 s, grey line: 1 min, yellow line: 2 min, blue line: 5 min, green line: 10 min). Residues comprising a region with significant differences in HDX are indicated. The peptides are arranged according to their position from N- to C-terminus (Supplementary Data 5, Table 2). A dotted grey line indicates the 98% CI as a threshold for significance. Regions showing a significant increased HDX are coloured in a red scale in the crystal structures according to their difference in relative fractional uptake normalized to the uptake of the maximally labelled state (the time point showing higher difference is considered for colouring). The region showing protection toward HDX is coloured in blue. In grey, regions for which no HDX information was retrieved.

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