Fig. 2: Comparison of HDX of His6-Hsp70 WT and W90F. | Communications Biology

Fig. 2: Comparison of HDX of His6-Hsp70 WT and W90F.

From: Conformational dynamics of free and membrane-bound human Hsp70 in model cytosolic and endo-lysosomal environments

Fig. 2

Difference plots illustrating the difference in HDX at pH 5.3 (a) and pH 7.4 (b) between Hsp70 WT and W90F over the measured time points (cyan line: 5 s, orange line: 15 s, grey line: 1 min, yellow line: 2 min, blue line: 5 min, green line: 10 min, light blue line: 100 min, pink line: 1000 min). Residues comprising a region with a significant difference in HDX are indicated. The peptides are arranged according to their position from N- to C-terminus (Supplementary Data 5, Table 2). Dotted grey lines indicate the 98% CI as a threshold for significance. Regions showing a significant difference in dynamics are coloured in red scale in the crystal structure of the NBD according to their difference in relative fractional uptake normalized to the maximally labelled state. The time point considered for colouring is indicated in the figure. In grey, regions for which no HDX information was retrieved.

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