Fig. 5: Binding model of Hsp70 to the lysosomal membrane at different lumen pH. | Communications Biology

Fig. 5: Binding model of Hsp70 to the lysosomal membrane at different lumen pH.

From: Conformational dynamics of free and membrane-bound human Hsp70 in model cytosolic and endo-lysosomal environments

Fig. 5

The blue spheres represent BMP head, while the grey spheres represent the zwitterionic phospholipids. At pH 5.3 Hsp70 keeps a native-like fold and the NBD becomes immersed in the membrane, whereas the SBD binds peripherally. The elements coloured in red depict the interacting regions. At a pH between 4.5 and 5, Hsp70 adopts a “molten globule” conformation and the NBD interaction to the membrane is mostly mediated by unspecific electrostatic interactions (indicated by red dots) to the anionic BMP.

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