Fig. 1: SERPINB3-KO cells are more sensitive to radiation and cisplatin-induced cell death.

a, b Western blot showing SERPINB3 in B3-WT and B3-KO cell lines. Percent Sytox-positive CRISPR-Control (B3-WT) and SERPINB3-KO (B3-KO) cells at indicated time points after 2, 10, or 30 Gy RT in SW756 (a) and 2, 4, or 10 Gy in HT3 (b). P-value displays sample variation for two-way ANOVA with replicates over time, n.s. not significant (bar = mean of triplicate wells, 2–4 fields of view per well, error bars = standard deviations, one representative of three biologic replicates shown). c Representative confocal fluorescent images of SW756- and HT3-B3-WT and -B3-KO Sytox (pseudo-colored green) and merge of Hoescht (pseudo-colored blue) and Sytox images at 84 h post-treatment. Scale bar indicates 50 µm. d, e Percent Sytox-positive cells following treatment with increasing doses of RT with or without cisplatin at 96 h in SW756 (d) and HT3 (e) background (bar = mean of triplicate wells, 2–4 fields of view per well, error bars = standard deviations, one representative of three biologic replicates shown). Student’s t-test p-values shown, n.s. = p-value not significant, * = p < 0.05, ** = p < 0.05 for all comparisons of B3-WT vs B3-KO, except where indicated. f, g Clonogenic survival of B3-WT or B3-KO cells treated with increasing doses of RT with or without cisplatin in SW756 (f) or HT3 (g) background. Individual data points from a representative experiment (one of three biologic replicates) with fit linear quadratic equation. DMF for 10% surviving fraction is shown relative to that of Control cells treated with vehicle (reference).