Fig. 3: Increased B3-KO cell death is not explained by cell cycle distribution or impaired DNA-damage repair. | Communications Biology

Fig. 3: Increased B3-KO cell death is not explained by cell cycle distribution or impaired DNA-damage repair.

From: SERPINB3 (SCCA1) inhibits cathepsin L and lysoptosis, protecting cervical cancer cells from chemoradiation

Fig. 3

a, c Representative histograms for DNA content determined by propidium iodide staining and flow cytometry analysis of HT3 (a) or SW756 (c) cells, with PI intensity on the x-axis. b, c Mean and standard deviation quantified cell cycle distribution for triplicate repeats of HT3 (b) and SW756 (d) experiments. Individual data points are shown, and asterisks indicate students t-test p-value < 0.05. e ɣH2AX foci per nucleus in HT3- and SW756-B3-WT and -B3-KO cells 30 min or 24 h after treatment with sham or 2 Gy. Dots represent individual nuclei, with mean and S.D. overlay. N.s. not significant, * = p < 0.05. f Representative nuclei shown for each condition with Hoechst 33342 (blue) and ɣH2AX (green) merge. White scale bars shown. g, h Western blot analysis of total cell lysates from HT3- and SW756-B3-WT and –B3-KO cells 30 min and 24 h after treatment with sham (S) or 2 Gy radiation. Band intensity of p-ATM normalized to band intensity of GAPDH is displayed under p-ATM blot.

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