Fig. 6: Radiation induces loss of lysosomal membrane integrity that precedes cell death. | Communications Biology

Fig. 6: Radiation induces loss of lysosomal membrane integrity that precedes cell death.

From: SERPINB3 (SCCA1) inhibits cathepsin L and lysoptosis, protecting cervical cancer cells from chemoradiation

Fig. 6

a, b Live-cell time-lapse confocal pseudocolor images of HT3-B3-KO (a) and HT3-B3-WT (b) cells at the indicated times after treatment with 10 Gy RT. LysoTracker deep red is pseudo-colored green and propidium iodide is pseudo-colored red. Scale bar = 25 μm. c, d cells shown were manually tracked over the timeframe indicated on the x-axis and intensity of LysoTracker deep red (LysoTr) and propidium iodide normalized to cell volume is plotted on the double y-axis. e Percent Sytox-positive HT3-B3-WT and –B3-KO cells treated with vehicle DMSO or E64d 24 h after sham or 10 Gy radiation. * = p < 0.05 by double-sided t-test. Each data point represents the % Sytox-positive quantified in 2–4 fields of view of one triplicate well (n = 3 per condition), and is a representative one of three biologic replicate experiments. Mean indicated by bar height and error bars show standard deviation.

Back to article page