Fig. 6: Polyglutamine expansion or deletion of exon 1 has modest effects on the full-length HTT-HAP40 SAXS profile. | Communications Biology

Fig. 6: Polyglutamine expansion or deletion of exon 1 has modest effects on the full-length HTT-HAP40 SAXS profile.

From: Huntingtin structure is orchestrated by HAP40 and shows a polyglutamine expansion-specific interaction with exon 1

Fig. 6

a SEC-MALS analysis of HTT-HAP40 samples Q23 (red), Q54 (blue) and Δexon 1 (green). b Experimental SAXS data. c Rg-based (dimensionless) Kratky plots of experimental SAXS data for HTT-HAP40 Q23 (red), Q54 (blue) and Δexon 1 (green). d Normalised pair distance distribution function P(r) calculated from experimental SAXS data with GNOM for HTT-HAP40 samples. e SAXS parameters for data validation and interpretation including radius of gyration (Rg) calculated using Guinier fit in the q range 0.015 < q < 0.025 Å−1, radius of gyration calculated using GNOM, maximum distance between atoms calculated using GNOM and the molecular mass estimated using SAXSMoW with expected masses from the respective construct sequences shown in the parentheses.

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