Table 1 Cryo-EM data collection, refinement and validation statistics.
HTT-HAP40 (EMDB-22106) (PDB 6X9O) | |
|---|---|
Data collection and processing | |
Magnification | 165,000 |
Voltage (kV) | 300 |
Electron exposure (e−/Å2) | 48.0 |
Defocus range (μm) | 1.2–3.0 |
Pixel size (Å) | 0.822 |
Symmetry imposed | C1 |
Initial particle images (no.) | 2,240,373 |
Final particle images (no.) | 647,468 |
Map resolution (Å) | 2.6 |
FSC threshold | 0.143 |
Map resolution range (Å) | 2.5–3.5 |
Refinement | |
Initial model used (PDB code) | 6EZ8 |
Model resolution (Å) | 2.6 |
FSC threshold | 0.143 |
Model resolution range (Å) | 2.5–3.5 |
Map sharpening B factor (Å2) | −42.3 |
Model composition | |
Non-hydrogen atoms | 20,899 |
Protein residues | 2669 |
Ligands | 0 |
B factors (Å2) | |
Protein | 47.14 |
Ligand | N/A |
R.m.s. deviations | |
Bond lengths (Å) | 0.006 |
Bond angles (°) | 1.102 |
Validation | |
MolProbity score | 2.11 |
Clashscore | 14.85 |
Poor rotamers (%) | 0.56 |
Ramachandran plot | |
Favoured (%) | 93.41 |
Allowed (%) | 6.29 |
Disallowed (%) | 0.30 |
