Fig. 2: Lipid-A transport by MsbA.
From: Energetics of lipid transport by the ABC transporter MsbA is lipid dependent
a Structural formula of biotin-Lipid-A containing four primary hydroxymyristate (C14) acyl chains and one secondary myristate and laurate (C12) acyl chain. The orange brackets highlight the two phosphate groups in the glucosamine moieties of the lipid. b Biotin-Lipid-A assays with MsbA-WT and substrate binding chamber mutant MsbA-TripRA (R78A R148A R296A). Data refer to different provisions of metabolic energy: (i) Control (no metabolic energy, pHin 6.8/pHout 6.8), (ii) ATP (pHin 6.8/pHout 6.8) or (iii) imposed ΔpH (pHin 6.8/pHout 8.0) plus ATP. Data represent observations in three experiments (n = 3) with independently prepared batches of proteoliposomes. Lines and error bars in the scatter dot plot refer to mean ± s.e.m. (one-way analysis of variance; ****P < 0.0001). Asterisks above the horizontal line refer to comparisons with the control without metabolic energy.
