Fig. 6: CECs from Arg2−/− mice have impaired immunoregulatory properties.
From: Potent but transient immunosuppression of T-cells is a general feature of CD71+ erythroid cells
a The levels of CD3ζ in spleen CD4+ (a) and CD8+ (b) T cells in control (n = 4) and anemic mice (n = 4) based on intracellular staining. Histograms show the fluorescence of CD3ζ-FITC in CD4+ (a) and CD8+ (b) T cells. P values were calculated with one-way ANOVA with Tukey’s post hoc test. c Proliferation of αCD3/αCD28-stimulated CD4+ T cells co-cultured with CECs isolated from NHA Arg2−/− mice or NHA wild-type Arg2+/+ mice at a 1:4 ratio (T cells:CECs). Representative proliferation histograms of αCD3/αCD28-stimulated CD4+ T cells co-cultured with CECs isolated from Arg2−/− mice or wild-type Arg2+/+ mice in the presence of ARGi or ROSi. Histograms show the fluorescence of CTV (CellTraceViolet)-V450. P values were calculated with one-way ANOVA with Bonferroni’s post hoc test. Data show means ± SD (a, b) or means ± SEM (c). Each point in a–c represents data from individual mice. n values are the numbers of mice used to obtain the data or the number of biological replicates in in vitro experiments. The source data underlying a–c are provided as a Supplementary Data 2 file.
