Fig. 9: Erythroleukemia-derived erythroid cell lines suppress T cells in an ARG- and ROS-dependent mechanism.
From: Potent but transient immunosuppression of T-cells is a general feature of CD71+ erythroid cells
a The levels of CD71 and CD235a in erythroleukemia-derived erythroid cell lines. b–d Proliferation of CTV-labeled CD4+ T cells triggered by αCD3/αCD28 co-cultured with K562 (b), HEL92.1.7 (c), and TF-I (d) erythroid cell lines at different ratios (T cells:CECs) (n = 3). P values were calculated with repeated measures ANOVA with Dunnett’s post hoc test. e Representative histograms of CTV-BV421 fluorescence of CD4+ T cells co-cultured with erythroid cells at a 1:2 ratio in the presence of ARGi (OAT-1746, 1.5 μM) and ROSi (NAC, 200 μM) (n = 2). Statistical analyses are provided in Supplementary Fig. 18a. f–h Proliferation of CTV-labeled CD8+ T cells triggered by αCD3/αCD28 co-cultured with K562 (f), HEL92.1.7 (g), or TF-I (h) erythroid cells at different ratios (n = 3). P values were calculated with repeated measures ANOVA with Dunnett’s post hoc test. i Representative histograms of CTV-BV421 fluorescence of CD8+ T cells co-cultured with erythroid cell lines at a 1:2 ratio in the presence of ARGi (OAT-1746, 1.5 μM) and ROSi (NAC, 200 μM) (n = 2). Statistical analyses are provided in Supplementary Fig. 18b. Data show means ± SEM. n values are the numbers of biological replicates in in vitro experiments. The source data underlying b–d, f–h are provided as a Supplementary Data 2 file.
