Fig. 1: The role of N-terminus in Vasa for Vasa-to-Vasa oligomerization process.
From: Heterogeneous dissociation process of truncated RNAs by oligomerized Vasa helicase
a Schematic sequence of recombinant Vasa and dN-Vasa. N-IDR and C-terminal regions are disordered. DEAD-box is in the helicase domain. b The distributions of the estimated number of Vasa molecules at a single spot of WT-Vasa (black, total 198 spots) and dN-Vasa (grey, total 113 spots). Stripe bars indicate the distribution of WT-Vasa in the presence of RNA (total 140 spots, see “Methods”). The molecular numbers of Vasa were calculated from the averaged intensity of a single TMR molecule (b, d, e). c Immunostaining of BmN4 cells. Arrows show the nuage where Vasa aggregate. Scale bars represent 10 µm. d An example trajectory of fluorescence intensity of Vasa. Numbers indicate the molecular number of Vasa calculated from the fluorescence intensity. e The distribution of the estimated number of Vasa molecules in association and dissociation. Association and dissociation molecules were calculated to be 2.13 ± 0.17 (R2 = 0.964, 388 events of 173 spots) and 2.48 ± 0.25 (R2 = 0.987, 439 events), respectively. f The cumulative distribution of the dwell time before association and dissociation. Time constants in association and dissociation were calculated to be 465.3 ± 34.4 s (R2 = 0.986, 282 events) and 385.1 ± 15.3 s (R2 = 0.991, 282 events), respectively. Insets show the corresponding histograms fitted with a single exponential curve.
