Fig. 1: Specific protein-protein interactions occur between galectin-1 and different chemokines.
From: Chemokines modulate glycan binding and the immunoregulatory activity of galectins
a Representative spot blot analysis. Indicated proteins were spotted onto nitrocellulose filter and following incubation with galectin-1 protein-protein interaction was revealed by staining with anti-galectin-1 antibody. Anginex (Ax) and anti-galectin-1 monoclonal antibody served as positive controls, bovine serum albumin (BSA) and anti-ICAM monoclonal antibody served as negative control. b Representative gel-shift analysis. Proteins (0.2 mg/mL) were incubated for 2 h in the presence or absence of cross-linking agent disuccinimidyl suberate (DSS). Standard Western blot with anti-galectin-1 polyclonal antibody. The arrowheads point towards a shift of galectin-1. c Similar as in b but in the presence or absence of excess heparin. d Similar as in b but in the presence or absence of excess β-lactose. e Similar as in b but in the presence of different cytokines or increasing concentrations of CXCL4.
