Fig. 3: Reciprocal IP.

a Enrichment of proteins by two independent IP–MS experiments. The top 25 highest enriched proteins in Exp #2 and Exp #1 are shown in the table. b–f IP was carried out with rabbit anti-ELMSAN1 (r-αELMS), rabbit ani-MDN1 (r-αMDN1), rabbit anti-MKI67 (r-αKI67), and rabbit anti-YTHDC2 (r-αTDC2) antibodies, respectively. IB of the immunoprecipitates was carried out with these antibodies and mouse monoclonal anti-GNL2 (m-αGNL2) antibody, respectively. The bands indicated by < appear to have occurred during the experimental process. g Coomassie blue staining of the immunoprecipitates. h A model of the complex consisting of MKI67, GNL2, YTHDC2, MDN1, and ELMSAN1. This model is derived from evaluation of the reciprocal IP yields (Supplementary Table 3).