Fig. 5: Migration of nucleolar proteins, MKI67, MDN1, and GNL2.
From: RNAmetasome network for macromolecule biogenesis in human cells
a A model of RNAmetasome network protein migration in HEK293T. Nucleolar proteins with MDN1 are maintained in the nucleolus by binding of MKI67 to the nucleolus periphery chromatin. The MDN1 complex migrates out of the nucleolus and recruits some proteins in the nucleoplasm, such as YTHDC2. b Depletion of GNL2 relaxes the interaction between MKI67 and MDN1 and accelerates migration of the MDN1 complex to the nucleoplasm. c Depletion of MKI67 accelerates migration of the MDN1 complex to the nucleoplasm. d Co-IP of GNL2 and MKI67 with the anti-MDN1 antibody in GNL2 depleted nuclear and the control extracts. The siRNA and the universal negative control siRNA were added at 40 nM.
