Fig. 6: RNAmetasome network of Hela.cl1.

a Scatter diagram of the RNAmetasome network proteins. X and Y axes show net spectral count (SC) of the HEK293T and the Hela.cl1 proteins immunoprecipitated with the anti-ELMSAN1 antibody. Net SC of HEK293T = average of total SC − average of four control SC; likewise, net SC of Hela.cl1 = total SC − control SC. Net SC of HEK293T and Hela.cl1 were 14 and 10, respectively. In this diagram, 631 proteins are plotted after the Hela.cl1’s values are normalized by factor 14/10. r = 0.47, P < 0.0001. b–d MDN1, MKI67, GNL2 level of HEK293T and Hela.cl1 nuclear extracts estimated by western blotting. e IB of proteins immunoprecipitated with the anti-ELMSAN1 antibody. All procedures for these experiments were carried out in parallel, which reveals that MDN1 is at an undetectable level. This ELMSAN1 IB image (far left) is flipped and presented in Fig. 1f (left). f High-intensity scanned image of e and GNL2.