Fig. 2: Normalized sequencing coverage of full Y and X chromosomes in samples from young and old rats.

a Dot plot of the normalized median sequencing coverage of ChrY compared to the age of the rats. The observed sequencing coverage of the Y and X chromosomes was standardized using an expected value calculated as half of the median autosomal sequencing coverage (both sex chromosomes are single copy in the male genome). Significant differences, Linear Model: lm[median.chrY.coverage ~ age], * indicates P < 0.05. b Boxplot of the normalized median sequencing coverage for the 22 chromosomes in rats (20 autosomes and two sex chromosomes). The observed coverage for each autosome was normalized by the median coverage across autosomes. Only half of the autosomal coverage was used to normalize values from Y and X chromosomes. Significant differences, Mann–Whitney U test against a distribution with a fixed median of 0 (similar to the expected value). P values were Benjamin–Hochberg corrected: *** indicates P < 0.001. Error bars, maximum and minimum values, excluding outliers. c Dot plot of the normalized median sequencing coverage of chromosome X compared to the age of the rats. The observed coverage of the Y and X chromosomes was standardized using half of the median autosomal coverage. Significant differences, Linear Model: lm[median.chrX.coverage ~ age]. Sample sizes are indicated in each plot (n value).