Fig. 4: flimGANE enables accurate  FRET characterization from low-photon-count data. | Communications Biology

Fig. 4: flimGANE enables accurate  FRET characterization from low-photon-count data.

From: Generative adversarial network enables rapid and robust fluorescence lifetime image analysis in live cells

Fig. 4

a Schematic of CFP-g-YFP FRET pair interacting with glucose and normalized excitation and emission spectra of CFP and YFP. b Intensity contrast and FLIM images of CFP generated by TD_LSE, TD_MLE, DFD_LSE and flimGANE before and after adding 2 mM glucose. c Energy transfer efficiency, E, versus  glucose concentration (error bars, standard deviation errors on the parameter estimate, n = 1507~6824 pixels). The flimGANE FRET data can be well fitted by a sigmoidal curve (R2 = 0.92).

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