Fig. 2: Scatter plot of the HP [13C]bicarbonate signal intensities normalized to the total sum of metabolites and corresponding initial reaction rate as a function of the injected dose of HP [1-13C]pyruvate.
Points for 32 experiments (16 fed; 16 fasted) are coded by nutritional state (purple circles: fed; pink triangles: fasted). a The fitted regression line of a heteroscedastic linear model is shown for each state. The p-values correspond to the two-sided Wald t-tests of equality of intercept and slopes. b The initial reaction rate V0 (in arbitrary units) was obtained by multiplying the normalized HP [13C]bicarbonate signal, which can be assumed to be proportional to an apparent pyruvate-to-bicarbonate conversion rate constant (kpyr-bic), with the corresponding substrate dose. The dotted line is a linear regression.
