Fig. 7: Modulation of postsynaptic neural activity by Interluminescence in vivo.
From: Selective control of synaptically-connected circuit elements by all-optical synapses
a Schematic of the in vivo Interluminescence configuration targeting somatosensory thalamic nuclei and SI Barrel cortex (left panel). Confocal image of a PV cell expressing the excitatory step-function opsin with an EYFP tag (green) along with thalamocortical axon terminals expressing the hPOMC1-26-sbGLuc with dTomato tag (red) and cell nuclei stained with DAPI (blue) (right panel). b The average bioluminescence signal with ±1 SEM in semi-opaque bands for the Opsin (+) and Opsin (−) group (n = 2 and n = 3 animals, respectively). The bioluminescence time series were obtained by averaging across a circular ROI (50 pixel/290 µm diameter) adjacent to the electrode insertion point in the acquired image series. c Time-frequency spectrograms averaged across all laminar electrode contacts for the Opsin (+) (left panel) and Opsin (−) (right panel) cohorts. Time zero refers to the onset of the bioluminescence signal. d Depth profiles of gamma-band power (dB) (80–100 Hz) across the electrode contacts for the Opsin (+) group (left panel) and Opsin (−) group (right panel). e Gamma-band power (dB) relative to baseline plotted against gamma event counts after baseline. Small circles are individual animals, averaged across the electrode contacts. Error bars are ±1 SEM across the electrode contacts for a given animal. Large squares are the mean values for the Opsin (+) group and Opsin (−) group. Error bars represent ±1 SEM across animals. N = 3 animals for each group. f Gamma-band power (dB) relative to baseline plotted against MUA percent change from baseline. Same conventions as in (e). N = 3 animals for each group.
