Fig. 3: MARK3 overexpression inhibits cell proliferation in HGSOC cell lines. | Communications Biology

Fig. 3: MARK3 overexpression inhibits cell proliferation in HGSOC cell lines.

From: The metabolic stress-activated checkpoint LKB1-MARK3 axis acts as a tumor suppressor in high-grade serous ovarian carcinoma

Fig. 3

a, b Colony formation assay in MARK3 doxycycline (DOX)-inducible OVCAR3 (a) and CaOV3 (b) cells. Error bars represent mean ± standard deviation (SD) of three biological replicates. Statistical analysis was performed using an unpaired Student’s t-test. c Cell viability assay in parental OVCAR3 and MARK3 DOX-inducible OVCAR3. To exclude the potential cytotoxic effects of DOX, we treated both parental OVCAR3 and MARK3 DOX-inducible OVCAR3 cells with DOX and confirmed that only MARK3 DOX-inducible OVCAR3 showed a significant decrease in cell viability. Error bars represent mean ± SD of three biological replicates. Statistical analysis was performed using an unpaired Student’s t-test. n.s.: not significant. d Cell viability assay for the transient overexpression of MARK3 in various types of HGSOC-like cell lines defined by genomic profiles, such as TP53 mutation and hyper CNA. EGFP expression plasmid is used as a control. Relative cell number shows the relative ratio of the number of cells when EGFP and MARK3 are overexpressed, respectively (EGFP = 1.0). Error bars represent mean ± SD from three biological replicates. Statistical analysis was performed using an unpaired Student’s t-test. e RNA-seq analysis. Volcano plot showing differentially expressed genes (DEGs) obtained by MARK3 overexpression in OVCAR3 cells of three biological replicates. f Results of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis and Gene Ontology (GO) analyses of the top 500 upregulated and downregulated DEGs. KEGG pathway analysis and GO analysis were performed on the top 500 downregulated and the top 500 upregulated DEGs separately. Downregulated DEGs are enriched in cell cycle pathway in KEGG pathways and cell-cell adhesion and cell proliferation in GO terms; upregulated DEGs are enriched in lysosome pathway in KEGG pathways and extracellular matrix organization and translational initiation in GO terms. A modified Fisher Exact P-value (EASE score) is shown.

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