Fig. 6: Colony appearance of strain B0, P24-resistant mutants and transformants, and spot test assays. | Communications Biology

Fig. 6: Colony appearance of strain B0, P24-resistant mutants and transformants, and spot test assays.

From: Characterization of phage resistance and phages capable of intestinal decolonization of carbapenem-resistant Klebsiella pneumoniae in mice

Fig. 6

a Colony appearances of B1-1 and B1-1::pmshA compared with B0. b Colony appearances of B1-2 and B1-2::pwcaJ compared with B0. c–f Spot test assays of P24 and P39 on B0, B1-1, B1-1::pmshA, and B1-2::pwcaJ, respectively. P24 formed a clear large clearing zone with 13–15 mm (diameter) halos and a 7–8 mm hole in the middle of the zone in semi-solid LB plates containing strain B0, B1-1::pmshA and B1-2::pwcaJ. P39 formed a 7 mm clearing zone without a halo in plates containing P24-resistant mutant B1-1. The 20 mm scale is indicated.

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