Fig. 1: Schematic diagram of the two target regions (gRNA1 and gRNA2) and the locations of the polymorphic SNP markers (M1-M34) used in the genotyping assays.
From: Demonstration of targeted crossovers in hybrid maize using CRISPR technology
Schematic SNP marker positions are shown as black, brown, and blue triangles. The blue triangles represent SNP markers that were shared in the two different genome editing experiments. The physical genome coordinates of the gRNA target sites are based on the B73 genome reference public assembly: Zm-B73-REFERENCE-NAM-5.0. (https://www.maizegdb.org)11. The physical position of each SNP marker can be found in Supplementary Data 3.
