Fig. 2: Experimental workflow to demonstrate guided homologous chromosome recombination in maize.
From: Demonstration of targeted crossovers in hybrid maize using CRISPR technology
a Two maize inbred lines were crossed to produce F1 hybrid material. b Isolated F1 embryo explants were separated into two groups for Agrobacterium -mediated transformation: (I) editing treatment; (II) control treatment. c, d The regenerated F1-T0 plants were reciprocally backcrossed to Parent B (the first experiment: gRNA1) or Parent A (the second experiment: gRNA2) for the identification of CO by genotyping. e The harvested BC1-F1 seeds were chipped to isolate a small amount of endosperm tissue for genotyping analysis using SNP TaqMan assays. f The genotyping results were examined for the presence of the expected reciprocal chromosomal recombination between the parental chromosomes.
