Fig. 3: Analysis of DNA editing patterns at the sites of targeted CO in the BC1-F1 plants identified in the genome editing experiments with gRNA1 and gRNA2 using Illumina sequencing.
From: Demonstration of targeted crossovers in hybrid maize using CRISPR technology
DNA was isolated from a single leaf disc sampled from each BC1-F1 plant for library construction and sequencing. Each bar on the graph represents a result derived from a single BC-F1 plant (n = 1). a The gRNA1 target site showing LbCas12a cutting pattern: the PAM sequence (red); gRNA target sequence (italic blue); a fragment of the downstream DNA sequence (black). b The graph shows the proportion of edited (blue) and non-edited (red) reads in 13 LbCas12a negative BC1-F1 plants with the targeted CO and two control plants. BC1-F1 plants selected for further characterization are highlighted with red stars (*) c The gRNA2 target site showing LbCas12a cutting pattern; d The graph shows the proportion of edited (blue) and non-edited (red) reads in 14 randomly selected LbCas12a negative BC1-F1 plants with targeted CO derived from F1-T0 by Parent A backcross.
