Table 1 Result of the genotyping analysis and identification of the BC1-F1 seeds with chromosomal recombination at the gRNA1 target site.
From: Demonstration of targeted crossovers in hybrid maize using CRISPR technology
Constructs | Transformation | Events# | Total seeds genotyped | Seeds with recombinations between M1-M33 556 kb region (%) | Seeds with recombinations between M8-M9 0.8 kb region (%) |
|---|---|---|---|---|---|
pCpf1_gRNA1 | Editing plasmid | 42 | 4200 | 135 (3%) | 30 (0.71%) |
pCpf1_gRNA_control | Control plasmid | 15 | 2265 | 69 (3%) | 1 (0.04%) |
