Table 2 The BC1-F1 seeds with chromosomal recombination identified at the gRNA1 target site grouped by F1-T0 plants (events).
From: Demonstration of targeted crossovers in hybrid maize using CRISPR technology
Transformation | Event # | Total seeds genotyped | Seeds with recombinations between M1-M33 556 kb region (%) | Seeds with recombinations between M8-M9 0.8 kb region (%) | LbCas12a negative seeds |
|---|---|---|---|---|---|
pCpf1_gRNA1 | Event 1 | 308 | 27 (9%) | 18 (6%) | 8 |
pCpf1_gRNA1 | Event 2 | 311 | 20 (6.5%) | 11 (3.5%) | 4 |
pCpf1_gRNA1 | Event 3 | 461 | 19 (4%) | 1 (0.2%) | 1 |
pCpf1_gRNA_control | Event 4 | 542 | 13 (2.4%) | 1 (0.1%) | Â |
pCpf1_gRNA_control | Event 5 | 543 | 15 (3%) | 0 | Â |
pCpf1_gRNA_control | Event 6 | 196 | 1 (0.5%) | 0 | Â |
