Table 3 Result of the genotyping analysis and identification of the BC1-F1 seeds with chromosomal recombination at the gRNA2 target site.
From: Demonstration of targeted crossovers in hybrid maize using CRISPR technology
Constructs | Transformation | Event# | Total seeds genotyped | Seeds with recombination between M3-M34 359 Kb region | Seeds with recombination between M25-M25 0.4 Kb region |
|---|---|---|---|---|---|
pCpf1_gRNA2 | Editing plasmid | 43 | 4798 | 197(4%) | 175(3.6%) |
pCpf1_gRNA_control | Control plasmid | 15 | 3370 | 37(1%) | 0 |
