Table 4 The BC1-F1 seeds with chromosomal recombination at the gRNA2 target site derived from a single F1-T0 event.
From: Demonstration of targeted crossovers in hybrid maize using CRISPR technology
Transformation | Event | Seeds with B/A recombination | Seeds with A/B recombination | Total seeds genotyped | Seeds with B/A recombination LbCas12a negative | Seeds with A/B recombination LbCas12a negative | Total Seeds LbCas12a negative |
|---|---|---|---|---|---|---|---|
pCpf1_gRNA2 | ZM_S22440456 | 79 | 96 | 175 | 34 | 57 | 91 |
