Table 5 Specific DNA editing patterns introduced by the gRNA1 at the site of the targeted CO in the analyzed BC1-F1 plants.
From: Demonstration of targeted crossovers in hybrid maize using CRISPR technology
BC1-F1 plant # | Construct | Edited reads (%) | Reads without mutation (%) | Editing pattern |
|---|---|---|---|---|
Event-1.4 | pCpf1_gRNA1 | 0 | 99 | TTTC(ATAGTACACGTCTGGGTAGATTC)ACAGTACACGTCCGGGTAGATCCTATGGAA |
Event-1.5 | pCpf1_gRNA1 | 61 | 38 | TTTC(A----------------------)-CAGTACACGTCCGGGTAGATCCTATGGAA |
Event-1.7 | pCpf1_gRNA1 | 55 | 44 | TTTC(ATAGTACACGTCTG---------)ACAGTACACGTCCGGGTAGATCCTATGGAA |
Event-1.8 | pCpf1_gRNA1 | 1 | 98 | TTTC(ATAGTACACGTCTGGGTAGATTC)ACAGTACACGTCCGGGTAGATCCTATGGAA |
Event-1.9 | pCpf1_gRNA1 | 0 | 99 | TTTC(ATAGTACACGTCTGGGTAGATTC)ACAGTACACGTCCGGGTAGATCCTATGGAA |
Event-1.11 | pCpf1_gRNA1 | 57 | 42 | TTTC(ATAGTACACGTC-----------)------------CGGGTAGATCCTATGGAA |
Event-1.14 | pCpf1_gRNA1 | 59 | 40 | TTTC(A----------------------)-CAGTACACGTCCGGGTAGATCCTATGGAA |
Event-1.18 | pCpf1_gRNA1 | 58 | 41 | TTTC(A----------------------)-CAGTACACGTCCGGGTAGATCCTATGGAA |
Event-2.2 | pCpf1_gRNA1 | 51 | 49 | TTTC(ATAGTACACGTCTGGGTAG---C)ACAGTACACGTCCGGGTAGATCCTATGGAA |
Event-2.6 | pCpf1_gRNA1 | 46 | 54 | TTTC(ATAGTACACGTCTGGGTAG---C)ACAGTACACGTCCGGGTAGATCCTATGGAA |
Event-2.10 | pCpf1_gRNA1 | 0 | 99 | TTTC(ATAGTACACGTCTGGGTAGATTC)ACAGTACACGTCCGGGTAGATCCTATGGAA |
Event-2.11 | pCpf1_gRNA1 | 0 | 99 | TTTC(ATAGTACACGTCTGGGTAGATTC)ACAGTACACGTCCGGGTAGATCCTATGGAA |
Event-3.1 | pCpf1_gRNA1 | 54 | 46 | TTTC(ATAGTACACGTCTGG--------)--------CGTCCGGGTAGATCCTATGGAA |
Event-4.1 | pCpf1_gRNA_control | 0 | 99 | TTTC(ATAGTACACGTCTGGGTAGATTC)ACAGTACACGTCCGGGTAGATCCTATGGAA |
Event-4.2 | pCpf1_gRNA_control | 0 | 99 | TTTC(ATAGTACACGTCTGGGTAGATTC)ACAGTACACGTCCGGGTAGATCCTATGGAA |
