Table 6 Specific DNA editing patterns introduced by the gRNA2 at the site of the targeted CO in the analyzed BC1-F1 plant.
From: Demonstration of targeted crossovers in hybrid maize using CRISPR technology
BC1-F1 Plant # | Construct | Edited reads (%) | Reads without mutation (%) | Editing pattern |
|---|---|---|---|---|
ZM_S22440456-1 | pCpf1_gRNA2 | 48 | 52 | TTTA(GACCTGACAGCCAGTC—AAAGC)GGATGGAGGGAATTTGCAATTTAGTTTGC |
ZM_S22440456-2 | pCpf1_gRNA2 | 48 | 52 | TTTA(GACCTGACAGCCAGTC—AAAGC)GGATGGAGGGAATTTGCAATTTAGTTTGC |
ZM_S22440456-3 | pCpf1_gRNA2 | 49 | 51 | TTTA(GACCTGACAGCCAGTC—AAAGC)GGATGGAGGGAATTTGCAATTTAGTTTGC |
ZM_S22440456-4 | pCpf1_gRNA2 | 47 | 53 | TTTA(GACCTGACAGCCAGTC—AAAGC)GGATGGAGGGAATTTGCAATTTAGTTTGC |
ZM_S22440456-5 | pCpf1_gRNA2 | 47 | 53 | TTTA(GACCTGACAGCCAGTC—AAAGC)GGATGGAGGGAATTTGCAATTTAGTTTGC |
ZM_S22440456-6 | pCpf1_gRNA2 | 48 | 51 | TTTA(GACCTGACAGCCAGTC—AAAGC)GGATGGAGGGAATTTGCAATTTAGTTTGC |
ZM_S22440456-7 | pCpf1_gRNA2 | 50 | 50 | TTTA(GACCTGACAGCCAGTC—AAAGC)GGATGGAGGGAATTTGCAATTTAGTTTGC |
ZM_S22440456-8 | pCpf1_gRNA2 | 49 | 51 | TTTA(GACCTGACAGCCAGTC—AAAGC)GGATGGAGGGAATTTGCAATTTAGTTTGC |
ZM_S22440456-9 | pCpf1_gRNA2 | 47 | 52 | TTTA(GACCTGACAGCCAGTC—AAAGC)GGATGGAGGGAATTTGCAATTTAGTTTGC |
ZM_S22440456-10 | pCpf1_gRNA2 | 50 | 50 | TTTA(GACCTGACAGCCAGTC—AAAGC)GGATGGAGGGAATTTGCAATTTAGTTTGC |
ZM_S22440456-11 | pCpf1_gRNA2 | 49 | 51 | TTTA(GACCTGACAGCCAGTC—AAAGC)GGATGGAGGGAATTTGCAATTTAGTTTGC |
ZM_S22440456-12 | pCpf1_gRNA2 | 49 | 50 | TTTA(GACCTGACAGCCAGTC—AAAGC)GGATGGAGGGAATTTGCAATTTAGTTTGC |
ZM_S22440456-13 | pCpf1_gRNA2 | 51 | 49 | TTTA(GACCTGACAGCCAGTC—AAAGC)GGATGGAGGGAATTTGCAATTTAGTTTGC |
ZM_S22440456-14 | pCpf1_gRNA2 | 49 | 51 | TTTA(GACCTGACAGCCAGTC—AAAGC)GGATGGAGGGAATTTGCAATTTAGTTTGC |
