Table 1 Net photosynthetic rates of the diatom Thalassiosira weissflogii grown and measured under four pO2/pCO2 conditions with and without the extracellular (periplasmic) carbonic anhydrase inhibitor acetazolamide (AZ).

From: Enhancement of diatom growth and phytoplankton productivity with reduced O2 availability is moderated by rising CO2

  

-AZ

 + AZ

Inhibition

  

(fmol O2 cell−1 min−1)

(fmol O2 cell−1 min−1)

(%)

AC

AO

41 ± 2.9b

36 ± 2.3cd

11 ± 1.6b

LO

45 ± 3.5b

37 ± 2.6c

18 ± 2.1a

HC

AO

43 ± 2.7b

44 ± 3.0b

None

LO

55 ± 2.1a

56 ± 2.3a

None

  1. Values represent means ± standard deviation (n = 3) for replicate cultures under ambient (AO, ~255 μM) and low O2 levels (LO, ~57 μM) with ambient (AC, ~15 μM) and elevated CO2 levels (HC, ~35 μM), and different letters (superscripted) represent significant differences (P < 0.05, LSD test) among +AZ and −AZ treatments. Photosynthetic inhibition in high CO2-grown cells was not detected (none), while low O2-grown cells showed the highest photosynthetic inhibition. The detailed information for the experimental features and timing points are shown in Supplementary Fig. 3.
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